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Comparing the Sensitivity of Visual Evoked Potential and Standard Achromatic Perimetry in Diagnosis of Optic Neuritis- Juniper Publishers

Juniper Publishers- JOJ Ophthalmology
Purpose: To evaluate the diagnostic power of Visual Evoked Potential (VEP) and Standard Achromatic Perimetry (SAP) in a group of patients with Multiple Sclerosis (MS) and a history of optic neuritis (ON).
Methods: 136 eyes of 68 patients (11 male and 57 female, average age 27.18 years) with a confirmed diagnosis of MS underwent SAP and pattern VEPs. 67 eyes (49.2%) had no history of ocular involvement and a negative ophthalmologic examination, while 69 had proven ON according to physical examination. The mean deviation (MD) and the pattern standard deviation (PSD) of perimetry and the amplitude & latency of VEP in both normal and abnormal eyes were recorded. MD & PSD<0.5% and pl00 latency more than 118 millisecond were considered abnormal.
Results: MD was abnormal in 93.9% of the eyes with ON, PSD was abnormal in 69.4% and latency was abnormal in 55% of the affected eyes with ON. In overall, VEP can diagnose 55% of the eyes affected with ON and SAP can diagnose 96%. In statistical analysis of MD, PSD, latency and amplitude values with Mann-Whitney Test MD and PSD were statistically significant (MD has p<0.001, PSD has P<0.05, latency has p=0.83 and amplitude has p=0.38).
Conclusion: The results of this study demonstrate that SAP is more sensitive than VEP in the diagnosis of ON. SAP is a less operator and patient dependent technique and the patient test reliability can be assessed with parameters such as False-positive response rate, False- negative response rate, Fixation loss & Short-term fluctuation measurement. Because of these advantages, SAP has better sensitivity than VEP in the diagnosis of ON. Our results suggest that clinical and subclinical visual involvement of ON can be better diagnosed using both SAP and VEP tests, together.
Keywords: Keywords: Diagnostic power of SAP & VEP; Multiple sclerosis


Multiple sclerosis (MS) is an autoimmune disease that involves the Optic Nerve, brain and spinal cord by damaging the myelin sheath [1]. The disease can affect patients' visual pathway and therefore lead to Optic Neuritis (ON) which is an eye discomfort accompanied by decreased visual acuity, changes in the visual field, uncontrollable rapid eye movements and double vision (clinical and frequent subclinical evident manifestations) [1-3]. De-myelinating disease leads to less responses of the brain and the optic nerve which is detected by the visual evoked.
potential [3-5]. Alterations in the visual evoked potential (VEP) and standard achromatic perimetry (SAP) have been reported to be useful in patients with MS. Many studies have shown the presence of abnormality in the results of VEP and SAP in optic neuritis [6], but there is little agreement about the prevalence of these abnormalities or about which examination is more sensitive [7-10]. In this article, our aim is to evaluate the diagnostic power of SAP & VEP in a group of patients with Multiple Sclerosis (MS) and a history of optic neuritis.

Material and Methods

Sixty eight patients with ON and MS were recruited from the neurology and neuro-ophthalmology clinics of Rassoul Akram hospital in 2010-2011. The diagnosis of ON/MS was made based on standard clinical symptoms, examination findings, and radiologic abnormalities. In all patients, the disease was in remission and no patient was affected by any general disease apart from MS [11].
The diagnosis of ON was based on clinical signs and symptoms; such as: history of a progressive decrease of vision, color vision deficit, painful eye movements, fundus examination and relative afferent pupillary defect (RAPD) which is diagnosed by a neurologist or neuro-ophthalmologist. Patients voluntarily participated in this study and all participants were enrolled after an informed consent was obtained. All patients underwent full ophthalmic examination, including best-corrected visual acuity (BCVA) measurement, slit lamp biomicroscopy, applantation tonometry, and fundus examination after pupillary dilation. VEP pattern reversal was performed (Sirius Galileo; Esaote Biomedica, Florence, Italy). Latency and amplitude were recorded and AP100 latency>118ms (i.e., 2SD above the mean) was considered abnormal.
Standard achromatic perimetry (SAP) was performed by means ofthe Humphrey Field Analyzer 750 (model 750; Carl Zeiss Meditec, Dublin, CA) using the 30-2 program with the Swedish interactive threshold algorithm (SITA) standard strategy. Two consecutive visual field examinations were performed for each patient, but only the second was evaluated for the purpose of the study and only if reliability indices were normal. The criteria for abnormality were mean deviation (MD) or pattern standard deviation (PSD) and were labeled abnormal by the instrument's software. VEP and SAP were done for all patients with one expert optometrist.
Patient data were collected through questionnaires, respectively, were entered into the SPSS software version 18.0 and were then analyzed. Maximum, minimum, and SD±Mean were reported for the quantitative data and for the qualitative data, the number (percentage) was stated. The Chi-Square test, with the aid of SPPS software was used to evaluate the relationship between qualitative variables and Mann-Whitney test was used for evaluation of normality between data and a P-value of<0.05 was considered valuable. VEP and SAP were done in all 68 patients (136 eyes). The age, sex, MD, PSD, amplitude and latency recorded are shown in Table 1. Results
From the 68 patients that were included in this study, 11 were male (16.2%) and 57 were female (83.8%). The mean±SD age of the patients was 27.18±8.5 (range:14-61 years old). 49.6% of the eyes that were included in the study were normal and 50.4% had ON. The mean MD value was -7.86db (with SD: 8.4) with a range of -34.49db to7.57db. Also, the mean±SD MD value in normal eyes was -4.89db±7.12 with a range of -34.49db and 7.57db, while the mean value±SD of MD in the affected eyes was-10.63db±8.98 with a minimum of -31.28db and a 5.18db maximum. MD was negative in 19.1 % of the normal eyes and positive in 80.9%, while being negative in 6.1% and positive in 93.9% of the eyes affected with ON. The mean±SD, PSD value was 4.82d3.6 with a minimum of 1.06db and a maximum of 14.46db. PSD value in normal eyes had a mean±SD of 3.55db±3.09 with a minimum and maximum of 1.06db and 14.13db; and the mean value of PSD in affected eyes was 5.78db (SD: ±3.75) with a minimum of 1.34db and a 14.13db maximum. PSD was negative in 59.6% and positive in 40.4% of the normal eyes and 30.6% negative and 69.4% positive in the affected eyes with ON.
The mean±SD latency value was 112.03ms±18.98 with a range of 58ms to 157ms. The mean±SD latency value in normal eyes was 109.98ms±17.03 with a range of 58ms to 157ms while in the affected eyes, the mean±SD value of latency was 114.07ms±19.85 with a 58ms minimum and a maximum of 150ms. Also, latency was negative in 70.6% and positive in 29.4% of the normal eyes, while being 45% negative and 55% positive in the affected eyes with ON.
The mean±SD amplitude value was 8.79μv±6.41 with the minimum of 0.5μv and a maximum of 34.9μv. The mean±SD amplitude value in normal eyes was 10.04μv±6.76 with a range of 1.13μv to 27.90μv and in the affected eyes, the mean±SD value of amplitude was 7.71μv±5.86 with a 0.50μv minimum and a maximum of 27.90μv.
In statistical analysis of MD, PSD, latency and amplitude P<0.05, that statistically significant but latency has p=0.83 and values with Mann-Whitney Test, MD has p<0.001, PSD has amplitude has p=0.38 (Table2).


In many studies, subclinical alterations can be helpful in diagnosing some cases of the visual system in both normal and Multiple Sclerosis patients, but we found that there was little agreement about the prevalence of these abnormalities [12-15] and the fact that which abnormality has the most sensitivity(6,14). In our study there was no single examination detected in all cases of visual involvement. So, the VEP P100 latency is the most diffuse and also the parameter of it had been used for detection the optic nerve involvement, but it is not very sensitive for the diagnosis of post chiasmal localizations. The results of our study had been impacted by other related studies too.
On other hand, the result of other studies shows that the VEP P100 latency cannot detect all cases of optic nerve involvement [10]. Also, some studies demonstrate that automated perimetery can too be an excellent tool in evaluating neuro-ophthalmologic disorders. Our studies show that SAP is more sensitive than VEP in the diagnosis of ON. In overall, VEP can diagnose 55% of the affected eyes with ON and SAP can diagnose 96%. In statistical analysis of MD, PSD, latency and amplitude values with Mann- Whitney Test, MD has p<0.001 and PSD has P<0.05 and latency has p=0.83 and amplitude has p=0.38. SAP and is a less operator and patient-dependent technique and the patient test reliability can be assessed with parameters such as False-positive response rate, False-negative response rate, Fixation loss & Short-term fluctuation measurement.
In an article by Ruseckaite R et al. [16], the results on Frequency doubling illusion VEPs and automated perimetry in Multiple Sclerosis is shown. The recordings in the study were obtained from 27 Normal subjects, 26MS patients who had experienced Optic Neuritis (MSON) and 24MS patients without a history of ON (MSNON). Ruseckaite R et al. [16] study also demonstrated that discriminant models based on the Frequency Doubling Technology (FDT) thresholds and multi focal VEP (mfVEPs) were able to diagnose more that 90% of MSON patients, but performed poorly for MSNON patients.
Because of these advantages, SAP can have better sensitivity than VEP in the diagnosis of ON. (96%vs55%). Other advantages of SAP are as follows:

  1. Standardized testing conditions, which allow better serial and inter- institutional comparisons of fields
  2. Less technician dependence which improves sensitivity.
  3. Producing numerical data that are amenable to statistical analysis for comparisons and clinical studies.
In VEP, if a patient loses focus on the target, the test results may be false negative or false positive. For Most clinical situations, the VEP is of limited use. It is subject to numerous factors that may produce abnormal waveforms in the absence of visual pathway damage, including uncorrected refractive error, media opacity, amblyopia, fatigue, and inattention (either intentional or unintentional). In Most cases, the VEP is unnecessary for the diagnosis of optic neuropathy and is less accurate to quantify it than perimetry [8,9]
The two scenarios in which VEPs remain clinically useful are: First, the evaluation of the integrity of the visual pathway in infants or inarticulate adults. In this case, a preserved flash or pattern response confirms intact pathways and an abnormal flash response consistently reflects gross impairment. An abnormal pattern response is less useful, as it may indicate damage or this may be a false- negative result. Second, confirming intact visual pathways in patients with markedly abnormal subjective visual responses of a suspected nonorganic origin [10,11].
Corallo G et al. [17], in a study about conventional perimetry and visual evoked potentials in the assessment of patients with Multiple Sclerosis, demonstrated that the group of asymptomatic subjects had abnormal Conventional Automated Perimetry (CAP) in 1 eye (6.25%), abnormal Short-wavelength automated perimetry (SWAP) in 9 (56.2%), abnormal FDT in 11 (68.7%), and abnormal VEPs in 7 (43.7%). Also, Corallo G et al. [17] show that the combined use of all techniques allowed the researchers to identify silent optic nerve impairment in 15 (93.7%) eyes of MS patients. In another study by Della et al. [18], on the nerve fiber layer analysis with GDx with a variable corneal compensator in patients with Multiple Sclerosis, the result is shown that GDx VCC is less able to detect early defects in MS patients compared to the currently used standard techniques of SAP and VEPs.
At last in our cases, intact pattern had been developed a measure of expected acuity when stimuli of various sizes and confirms an intact visual pathway. Because voluntary inattention or de focusing may markedly reduce the pattern waveform, again, an abnormal or absent pattern response does not confirm organic disease.


According to the results of this study and what was discussed above, we suggest using both VEP and SAP tests for the diagnosis and follow up of Optic Neuritis in patients with Multiple Sclerosis.


a) The manuscript is original work of author. All data, tables, figures, etc. used in the manuscript are prepared originally by authors, otherwise the sources are cited and reprint permission is attached.
b) The manuscript has not been and will not be published elsewhere or submitted elsewhere for publication.
c) Authors mention that there is no conflict of interest in this study.
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Demographic Factors, Forensic Profile, Substance Abuse and Crime in Violent Offenders at a Maximum Security Prison in North Central Nigeria. - Juniper Publishers

Demographic Factors, Forensic Profile, Substance Abuse and Crime in Violent Offenders at a Maximum Security Prison in North Central Nigeria. - Juniper Publishers

Demographic Factors, Forensic Profile, Substance Abuse and Crime in Violent Offenders at a Maximum Security Prison in North Central Nigeria.
Background: Violent criminal behavior has been linked to multiple factors among which are the abuses of alcohol/drugs, resulting in various prison terms. The relationship between violent criminal behavior and abuse of alcohol/drugs continue to be an area of debate with the two being related through shared risk-factors. The study aimed at exploring whether substance abuse in violent offenders occur frequently with criminal behavior and any specific demographic factors and/or forensic profile using secondary data.
Method: The study was archival cross-sectional descriptive in nature. Secondary data of 83 convicted violent-offenders was extracted from the primary database. Data was arranged serially (1- 83) and exported into the statistical package for social science (SPSS) version 21.0 for analysis.
Result:Alcohol was the single most abused substance (24.1%), followed by marijuana (7.2%). Alcohol was significantly associated with murder (χ2 6.125, P= 0.013) while marijuana and cocaine abuse were associated with murder and armed-robbery crimes (χ2 11.879, P= 0.001; χ2 20.512, P= 0.001), (χ2 58.242, P= 0.001; χ2 63.674, P= 0.001) respectively. Age and educational status were significantly associated with alcohol/drug abuse in subjects studied. However, using logistic regression substance abuse/dependence did not predict any significant-association with all the parameters studied in the subjects.
Conclusion:Alcohol/drugs abuse was significantly associated with criminal behaviors,demographic-variable and forensic-profile of subjects studied, however using logistic regression alcohol/drugs abuse didn’t predict any significant association with criminal behavior, demographic factors or forensic profile of subjects. - Juniper Publishers
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